Effect Of Blood Collection Tubes On Plasma Quality

Article

Apr 6

When it comes to collecting blood samples for laboratory testing, the type of blood collection tube used can have a significant impact on the quality of the plasma obtained. Different types of tubes are designed to preserve the integrity of the blood sample and ensure accurate test results. In this blog post, we will explore the effect of blood collection tubes on plasma quality and why it is important to choose the right tube for the job.

Types of Blood Collection Tubes

There are several different types of blood collection tubes available, each designed for specific tests and purposes. Some of the most common types include:

1. EDTA Tubes

EDTA tubes contain the anticoagulant ethylenediaminetetraacetic acid, which prevents blood from clotting by binding to calcium ions. These tubes are commonly used for hematology studies and are ideal for preserving cell morphology, making them a good choice for tests that require whole blood samples.

2. Serum Separator Tubes

Serum separator tubes contain a gel that separates the serum from the clot after the blood has been centrifuged. These tubes are used for chemistry tests that require serum samples, as the gel barrier prevents contamination of the serum with cells and other components of whole blood.

3. Citrate Tubes

Citrate tubes contain sodium citrate, which is an anticoagulant that binds to calcium ions and prevents blood from clotting. These tubes are commonly used for coagulation studies and tests that require plasma samples.

Effect of Blood Collection Tubes on Plasma Quality

The type of blood collection tube used can have a significant impact on the quality of the plasma obtained. Factors such as the type of anticoagulant used, the presence of additives, and the presence of contaminants can affect the results of laboratory tests. Here are some of the ways in which blood collection tubes can affect plasma quality:

1. Hemolysis

Hemolysis is the rupture of red blood cells, which can release hemoglobin and other cellular components into the plasma. Hemolyzed samples can affect the accuracy of test results, as they can interfere with the measurement of analytes such as potassium, magnesium, and lactate dehydrogenase. The choice of blood collection tube can influence the likelihood of hemolysis occurring, as some tubes are more prone to hemolysis than others.

2. Contamination

Contamination of the plasma sample with cells, proteins, or other components of whole blood can affect the accuracy of test results. Serum separator tubes are designed to prevent contamination of the serum with cells, while EDTA tubes are ideal for preserving cell morphology and preventing contamination of the sample with cell fragments. Choosing the right type of tube for the test being performed is important to ensure accurate results.

3. Anticoagulant Effects

The type of anticoagulant used in the blood collection tube can affect the quality of the plasma obtained. While some anticoagulants, such as EDTA and citrate, are effective at preventing blood from clotting, others may be less effective and can lead to clot formation. Proper mixing of the blood sample with the anticoagulant is also important to ensure that the sample is properly preserved and does not clot during processing.

Choosing the Right Blood Collection Tube

When selecting a blood collection tube for a specific test, it is important to consider the requirements of the test and the potential impact of the tube on the quality of the plasma sample. Here are some tips for choosing the right tube:

Consider the test requirements: Different tests require different types of samples, such as whole blood, serum, or plasma. Choose a tube that is designed to preserve the integrity of the sample for the test being performed.
Avoid using expired tubes: Check the expiration date of the blood collection tube before use, as expired tubes may not preserve the sample properly and can lead to inaccurate test results.
Follow proper handling and storage procedures: Store blood collection tubes according to the manufacturer's instructions and handle them carefully to prevent sample contamination or hemolysis.

By following these tips and choosing the right blood collection tube for the job, you can help ensure the quality and accuracy of the plasma samples obtained for laboratory testing.

Conclusion

The type of blood collection tube used can have a significant impact on the quality of the plasma obtained for laboratory testing. Factors such as hemolysis, contamination, and anticoagulant effects can affect the accuracy of test results and the reliability of the sample. By choosing the right blood collection tube for the test being performed and following proper handling and storage procedures, you can help ensure the quality and integrity of the plasma sample and obtain accurate test results.

References:

Disclaimer: The content provided on this blog is for informational purposes only, reflecting the personal opinions and insights of the author(s) on phlebotomy practices and healthcare. The information provided should not be used for diagnosing or treating a health problem or disease, and those seeking personal medical advice should consult with a licensed physician. Always seek the advice of your doctor or other qualified health provider regarding a medical condition. Never disregard professional medical advice or delay in seeking it because of something you have read on this website. If you think you may have a medical emergency, call 911 or go to the nearest emergency room immediately. No physician-patient relationship is created by this web site or its use. No contributors to this web site make any representations, express or implied, with respect to the information provided herein or to its use. While we strive to share accurate and up-to-date information, we cannot guarantee the completeness, reliability, or accuracy of the content. The blog may also include links to external websites and resources for the convenience of our readers. Please note that linking to other sites does not imply endorsement of their content, practices, or services by us. Readers should use their discretion and judgment while exploring any external links and resources mentioned on this blog.

Lauren Davis, BS, CPT

Lauren Davis is a certified phlebotomist with a Bachelor of Science in Public Health from the University of Miami. With 5 years of hands-on experience in both hospital and mobile phlebotomy settings, Lauren has developed a passion for ensuring the safety and comfort of patients during blood draws. She has extensive experience in pediatric, geriatric, and inpatient phlebotomy, and is committed to advancing the practices of blood collection to improve both accuracy and patient satisfaction.

Lauren enjoys writing about the latest phlebotomy techniques, patient communication, and the importance of adhering to best practices in laboratory safety. She is also an advocate for continuing education in the field and frequently conducts workshops to help other phlebotomists stay updated with industry standards.