Importance Of Order Of Draw

When it comes to drawing blood samples from patients, there is a specific sequence that must be followed in order to ensure accurate results and patient safety. This sequence is known as the Order of Draw, and it is a crucial aspect of phlebotomy practice. In this article, we will discuss the importance of the Order of Draw and why it is essential for healthcare professionals to adhere to this protocol.

What is Order of Draw?

The Order of Draw refers to the sequence in which blood samples are collected into different types of tubes during venipuncture. Each tube contains specific additives or chemicals that help preserve the blood sample for various tests. By following a specific order when collecting blood samples, healthcare professionals can prevent cross-contamination between tubes and ensure the accuracy of test results.

Why is Order of Draw Important?

The Order of Draw is important for several reasons:

1. Prevents cross-contamination: By following a specific sequence, healthcare professionals can prevent additives from one tube contaminating the sample in the next tube.
2. Ensures accurate test results: Each tube is designed to preserve the blood sample for specific tests. By collecting samples in the correct order, healthcare professionals can ensure that the integrity of each sample is maintained.
3. Enhances patient safety: Adhering to the Order of Draw helps prevent errors that could lead to misdiagnosis or inappropriate treatment for patients.

What is the Correct Order of Draw?

The Order of Draw may vary slightly depending on the laboratory and the specific tests being conducted. However, a general guideline for the correct sequence is as follows:

1. Yellow or SPS tubes (blood culture bottles): These tubes are used for collecting blood cultures to detect the presence of bacteria in the bloodstream. Collecting these tubes first helps prevent contamination of other samples.
2. Light blue tubes: These tubes contain sodium citrate, which is used for coagulation studies. By collecting these tubes second, the blood can begin to clot before testing.
3. Red or red-gray tubes: These tubes typically do not contain additives and are used for various tests, including blood chemistry and serology. Collecting these tubes third helps prevent interference from the additives in other tubes.
4. Gold or tiger-top tubes: These tubes contain a gel separator that helps separate serum from the blood cells. They are typically used for chemistry tests. Collecting these tubes fourth ensures proper separation of serum.
5. Light green, dark green, or mint green tubes: These tubes contain heparin or other anticoagulants and are used for chemistry tests. Collecting these tubes fifth helps prevent contamination from other additives.

Additional Tips for Following the Order of Draw

In addition to following the correct sequence for collecting blood samples, healthcare professionals should also follow these tips to ensure the accuracy of test results:

1. Verify the correct order with the laboratory: If you are unsure of the correct Order of Draw for a specific test, consult with the laboratory or refer to the laboratory manual for guidance.
2. Properly label each tube: Ensure that each tube is properly labeled with the patient's information, including name, date of birth, and medical record number.
3. Use the correct needle and equipment: Use the appropriate needle size and equipment for collecting blood samples to minimize patient discomfort and reduce the risk of hemolysis.

Conclusion

The Order of Draw is a critical aspect of phlebotomy practice that plays a significant role in ensuring the accuracy of test results and patient safety. By following a specific sequence when collecting blood samples, healthcare professionals can prevent cross-contamination between tubes, ensure the integrity of each sample, and enhance the quality of care provided to patients. It is essential for healthcare professionals to be knowledgeable about the correct Order of Draw and to follow this protocol diligently in their practice.

For more information on the importance of Order of Draw in phlebotomy practice, please visit ASCLS website.

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