Order Of Draw Importance For Accurate And Reliable Lab Results

Summary

• Incorrect order of draw can lead to Cross-Contamination of blood samples
• Improper technique can cause hemolysis, which affects Test Results
• Following the correct order of draw is crucial for accurate and reliable lab results

Introduction

When it comes to collecting blood samples for laboratory testing, following the proper order of draw is essential. The order of draw refers to the sequence in which different blood tubes are filled during Venipuncture. This process is crucial for ensuring the accuracy and reliability of the Test Results. Failing to adhere to the correct order of draw can lead to potential complications that can affect the quality of the samples and the accuracy of the Test Results.

What is the Order of Draw?

The order of draw is a standardized protocol for collecting blood samples in a specific sequence to minimize the risk of sample contamination. Following the correct order of draw helps prevent additives from one tube from contaminating subsequent tubes, ensuring accurate and reliable Test Results.

The Correct Order of Draw

1. Yellow tube - Blood Cultures
2. Light Blue tube - Citrate tubes for coagulation studies
3. Red tube - Tubes without additives or for serum separation
4. Gold or Tiger Top tube - Serum separator tubes with or without gel
5. Green tube - Heparin or lithium Heparin tubes
6. Lavender tube - EDTA tubes for hematology tests
7. Gray tube - Oxalate/fluoride tubes for glucose testing

Potential Complications of Not Following the Order of Draw

Failure to follow the correct order of draw can result in several potential complications that can impact the quality and accuracy of the blood samples collected for testing. Some of the potential issues that may arise include:

Cross-Contamination

One of the main risks of not following the order of draw is the potential for Cross-Contamination of blood samples. When tubes with additives are not drawn in the correct order, anticoagulants or other additives from one tube can contaminate subsequent tubes, leading to inaccurate Test Results. This can be particularly problematic for coagulation studies or other tests that require specific additives in the collection tube.

Hemolysis

Another complication that can arise from not following the proper order of draw is hemolysis, or the breakdown of red blood cells. Improper technique or mixing of blood samples can cause hemolysis, which can release potassium and other intracellular components into the plasma, affecting the accuracy of certain tests. Hemolysis can skew Test Results and lead to incorrect diagnoses or treatment decisions.

Incorrect Test Results

Failure to follow the correct order of draw can ultimately result in incorrect Test Results, which can have serious implications for patient care. Inaccurate Test Results can lead to misdiagnosis, inappropriate treatment decisions, or a delay in necessary medical interventions. Following the proper order of draw is crucial for ensuring that laboratory tests provide accurate and reliable information to Healthcare Providers.

Conclusion

Following the correct order of draw during Venipuncture is essential for ensuring the accuracy and reliability of laboratory Test Results. Failure to adhere to the proper order of draw can lead to Cross-Contamination of blood samples, hemolysis, and incorrect Test Results, all of which can impact patient care. Healthcare Providers and laboratory professionals must be diligent in following the established protocols for blood sample collection to minimize the risk of complications and ensure the quality of the Test Results.

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