The Importance Of The Correct Order Of Draw In Phlebotomy

Summary

• The correct order of draw is crucial in phlebotomy to prevent sample contamination and ensure accurate Test Results
• Following the correct order of draw helps maintain Sample Integrity and reduces the risk of Cross-Contamination
• Understanding the significance of the correct order of draw in phlebotomy is essential for all healthcare professionals involved in specimen collection

The Importance of the Correct Order of Draw in Phlebotomy

Phlebotomy is the process of collecting blood samples from patients for diagnostic testing. It is a critical step in the healthcare system as laboratory Test Results play a significant role in the diagnosis and treatment of various medical conditions. To ensure the accuracy and reliability of these Test Results, it is essential to follow the correct order of draw during blood collection. In this article, we will explore the significance of the correct order of draw in phlebotomy and its impact on patient care.

What is the Order of Draw?

The order of draw refers to the sequence in which different blood collection tubes should be filled during Venipuncture. Each tube contains specific additives or clot activators to preserve the integrity of the blood sample and prevent contamination. By following the correct order of draw, phlebotomists can minimize the risk of sample hemolysis, clot formation, and Cross-Contamination, ensuring the accuracy of Test Results.

Significance of the Correct Order of Draw

1. Prevents Contamination: One of the primary reasons for following the correct order of draw is to prevent sample contamination. Blood collection tubes are color-coded based on the additives they contain, and mixing them up can lead to inaccurate Test Results. For example, if a tube containing an Anticoagulant is drawn after a Clot Activator tube, it can affect the coagulation Test Results by interfering with the clotting process.

2. Ensures Sample Integrity: Following the correct order of draw helps maintain the integrity of the blood sample throughout the collection process. Additives in the collection tubes are designed to preserve the stability of different analytes, such as electrolytes, glucose, and enzymes. Mixing up the order of draw can compromise the quality of the sample and result in inaccurate Test Results.

3. Reduces Cross-Contamination: Cross-Contamination occurs when additives from one tube contaminate subsequent samples, leading to false results. By adhering to the correct order of draw, phlebotomists can minimize the risk of Cross-Contamination between tubes and ensure the accuracy of all Test Results. This is especially important in settings where multiple samples are collected from the same patient.

Common Order of Draw Sequence

While the specific order of draw may vary depending on the facility and testing requirements, a common sequence is as follows:

1. Yellow (Sterile): Used for blood cultures to detect bacterial or fungal infections

2. Light Blue (Sodium Citrate): Used for coagulation studies such as PT/INR and APTT

3. Red (No Additive or Clot Activator): Used for routine chemistry tests and serology

4. Gold or Tiger Top (Serum Separator Tube): Contains a Clot Activator and gel separator for chemistry tests

5. Green (Heparin): Used for electrolyte and enzyme tests

6. Lavender (EDTA): Used for hematology studies and blood bank tests

7. Gray (Sodium Fluoride/Potassium Oxalate): Used for glucose testing and some Toxicology screens

Conclusion

The correct order of draw is a critical aspect of phlebotomy that ensures the accuracy and reliability of laboratory Test Results. By following the appropriate sequence during blood collection, healthcare professionals can maintain Sample Integrity, prevent contamination, and reduce the risk of Cross-Contamination. Understanding the significance of the correct order of draw is essential for all individuals involved in specimen collection to provide high-quality patient care and accurate diagnostic results.

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