Proper Collection Techniques for Gold Top (SST) and Red Top Tubes: Ensuring Accurate and Reliable Test Results

Summary

• Gold Top (SST) and Red Top tubes are commonly used for blood sample collection
• Samples collected in Gold Top tubes are used for chemistry testing, while samples collected in Red Top tubes are used for serology testing
• Proper collection techniques must be followed to ensure accurate and reliable Test Results

Introduction

Gold Top (SST) and Red Top tubes are two of the most commonly used tubes for blood sample collection in the medical field. These tubes are used to collect blood samples for various types of testing, including chemistry and serology testing. In this article, we will explore how samples are collected in both Gold Top (SST) and Red Top tubes, as well as the differences between the two types of tubes.

Collection of Samples in Gold Top (SST) Tubes

Gold Top tubes, also known as serum separator tubes (SST), are used for chemistry testing. These tubes contain a gel separator that separates serum from the blood cells after centrifugation. Here are the steps for collecting samples in Gold Top tubes:

1. Prepare the patient: Verify the patient's identity and explain the procedure to them.
2. Locate the vein: Choose a suitable vein for Venipuncture and clean the area with an alcohol swab.
3. Perform Venipuncture: Insert the needle into the vein and collect the required amount of blood into the Gold Top tube.
4. Mix the sample: Gently invert the tube several times to ensure proper mixing of the blood with the gel separator.
5. Label the tube: Label the tube with the patient's information, including their name and date of collection.
6. Transport the sample: Store the sample at room temperature until it can be centrifuged to separate the serum from the blood cells.
7. Centrifuge the sample: After allowing the blood to clot, centrifuge the tube to separate the serum from the clot.
8. Transfer the serum: Carefully transfer the serum into a separate tube for testing, making sure not to disturb the gel separator.

Collection of Samples in Red Top Tubes

Red Top tubes are used for serology testing, which involves testing for antibodies in the blood. Unlike Gold Top tubes, Red Top tubes do not contain a gel separator. Here are the steps for collecting samples in Red Top tubes:

1. Prepare the patient: Verify the patient's identity and explain the procedure to them.
2. Locate the vein: Choose a suitable vein for Venipuncture and clean the area with an alcohol swab.
3. Perform Venipuncture: Insert the needle into the vein and collect the required amount of blood into the Red Top tube.
4. Allow the blood to clot: Let the blood sit undisturbed in the tube to allow it to clot.
5. Label the tube: Label the tube with the patient's information, including their name and date of collection.
6. Transport the sample: Store the sample at room temperature until it can be processed for serology testing.
7. Centrifuge the sample (optional): Depending on the specific test being performed, the sample may need to be centrifuged to separate the serum from the blood cells.

Differences Between Gold Top (SST) and Red Top Tubes

While both Gold Top (SST) and Red Top tubes are used for blood sample collection, there are some key differences between the two types of tubes:

Composition:

Gold Top (SST) tubes contain a gel separator that separates serum from blood cells, while Red Top tubes do not contain a gel separator.

Use:

Gold Top tubes are used for chemistry testing, while Red Top tubes are used for serology testing.

Processing:

Samples collected in Gold Top tubes must be centrifuged to separate the serum from the blood cells, while samples collected in Red Top tubes may or may not require centrifugation depending on the specific test being performed.

Conclusion

Proper sample collection techniques are essential to ensure accurate and reliable Test Results. Whether collecting samples in Gold Top (SST) or Red Top tubes, it is important to follow the correct procedures to avoid contamination or errors in the testing process. By understanding the differences between these two types of tubes and how samples are collected in each, Healthcare Providers can ensure the quality of the samples and the accuracy of the Test Results.

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