Proper Storage of Blood Samples: Guidelines for Maintaining Sample Integrity and Accuracy

Summary

  • Proper storage of blood samples is crucial to maintain Sample Integrity and accuracy in medical laboratory testing.
  • Different types of blood samples require specific temperature and duration guidelines for storage to prevent degradation.
  • Following recommended storage protocols can help ensure reliable Test Results and improve patient care in healthcare settings.

Introduction

Accuracy in medical laboratory testing is essential for diagnosing and treating patients effectively. Proper storage of blood samples is a critical aspect of maintaining Sample Integrity and ensuring accurate Test Results. In the United States, there are specific guidelines for storing different types of blood samples in healthcare settings to prevent degradation and maintain sample quality.

Recommended Temperatures and Durations for Storing Blood Samples

Whole Blood

Whole blood samples should be stored at controlled room temperature (20-25°C) or refrigerated at 2-8°C, depending on the testing requirements. The recommended duration for storing whole blood samples is typically 2-4 hours at room temperature or up to 24 hours if refrigerated. It is important to avoid freezing whole blood samples as it can cause hemolysis and affect Test Results.

Serum

Serum samples should be stored at room temperature (20-25°C) or refrigerated at 2-8°C, depending on the stability of the analytes being tested. The recommended duration for storing serum samples is typically 24 hours at room temperature or up to 48 hours if refrigerated. It is essential to avoid freezing serum samples as it can cause protein denaturation and affect Test Results.

Plasma

Plasma samples should be stored at room temperature (20-25°C) or frozen at -20°C or lower, depending on the testing requirements. The recommended duration for storing plasma samples is typically 24 hours at room temperature or up to 7 days if frozen. It is crucial to avoid repeated freeze-thaw cycles as it can lead to sample degradation and affect Test Results.

Buffy Coat

The buffy coat layer of a blood sample, which contains white blood cells and platelets, should be isolated and stored at room temperature (20-25°C) or refrigerated at 2-8°C. The recommended duration for storing the buffy coat layer is typically 24 hours at room temperature or up to 48 hours if refrigerated. It is important to avoid freezing the buffy coat layer as it can affect cell viability and Test Results.

Red Blood Cells

Red blood cells should be separated from whole blood samples and stored at refrigerated temperatures (2-8°C). The recommended duration for storing red blood cells is typically up to 42 days, depending on the type of preservative used. It is essential to follow specific guidelines for red blood cell storage to maintain cell integrity and oxygen-carrying capacity.

Platelets

Platelets should be separated from whole blood samples and stored at room temperature (20-25°C) with gentle agitation to prevent clotting. The recommended duration for storing platelets is typically up to 5 days, depending on the storage conditions and platelet quality. It is crucial to monitor platelet function and viability during storage to ensure optimal transfusion outcomes.

Conclusion

Proper storage of blood samples is vital for maintaining Sample Integrity and ensuring accurate Test Results in medical laboratory settings. Different types of blood samples require specific temperature and duration guidelines for storage to prevent degradation and maintain sample quality. By following recommended storage protocols, Healthcare Providers can improve patient care and optimize laboratory testing outcomes.

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