The Importance of Order of Draw in Blood Sample Collection: Best Practices for Healthcare Providers

Written By Jessica Turner, BS, CPT

Summary

  • Ensure patient comfort and safety during blood sample collection
  • Follow standard guidelines for order of draw to prevent contamination
  • Properly label and transport blood samples to maintain integrity

Introduction

When it comes to collecting blood samples in a medical lab setting in the United States, following the standard guidelines for the order of draw is crucial. This process helps to minimize the risk of sample contamination and ensure accurate Test Results. In this article, we will discuss the importance of the order of draw, the standard guidelines to follow, and best practices for collecting blood samples in home healthcare, clinical labs, and hospitals.

Importance of Order of Draw

The order of draw refers to the sequence in which blood samples are collected during a Venipuncture procedure. Following the correct order is essential to prevent Cross-Contamination between tubes and ensure accurate Test Results. When blood is drawn into multiple tubes, different additives are present in each tube to preserve the integrity of specific tests. If the tubes are not filled in the correct order, additives from one tube can contaminate the sample in the next tube, leading to inaccurate results.

Standard Guidelines for Order of Draw

1. Yellow (SPS) Tubes

  1. Yellow (SPS) tubes are typically used for blood cultures to detect the presence of bacteria.
  2. These tubes should always be drawn first to prevent any contamination from other additives.

2. Light Blue Tubes

  1. Light blue tubes contain Sodium Citrate and are used for coagulation studies.
  2. These tubes should be drawn after yellow tubes to avoid any interference with the coagulation Test Results.

3. Red or Tiger-Top Tubes

  1. Red or tiger-top tubes are used for chemistry tests that require serum.
  2. These tubes should be drawn after light blue tubes but before green or lavender tubes.

4. Serum Separator (Green or Gold) Tubes

  1. Green or gold tubes contain a gel separator and are used for chemistry tests that require serum.
  2. These tubes should be drawn after red or tiger-top tubes but before lavender tubes.

5. Lavender Tubes

  1. Lavender tubes contain EDTA and are used for hematology tests.
  2. These tubes should be drawn last to prevent contamination from other additives.

Best Practices for Blood Sample Collection

In addition to following the standard guidelines for the order of draw, it is essential to adhere to best practices for blood sample collection to ensure accurate and reliable Test Results:

  1. Verify patient identification before collecting blood samples to prevent errors.
  2. Use proper technique and equipment to minimize patient discomfort and reduce the risk of complications.
  3. Label tubes accurately with the patient's information and the date and time of collection.
  4. Transport blood samples to the lab promptly and store them appropriately to maintain Sample Integrity.
  5. Ensure Proper Disposal of sharps and biohazard waste to prevent accidents and contamination.

Conclusion

Following the standard guidelines for the order of draw is essential for collecting blood samples in a medical lab setting in the United States. By adhering to the correct sequence of tube collection, Healthcare Providers can minimize the risk of sample contamination, ensure accurate Test Results, and maintain patient safety. It is crucial to educate healthcare professionals on the importance of the order of draw and promote best practices for blood sample collection to improve the quality of care provided in home healthcare, clinical labs, and hospitals.

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Jessica Turner, BS, CPT

Jessica Turner is a certified phlebotomist with a Bachelor of Science in Health Sciences from the University of California, Los Angeles. With 6 years of experience in both hospital and private practice settings, Jessica has developed a deep understanding of phlebotomy techniques, patient interaction, and the importance of precision in blood collection.

She is passionate about educating others on the critical role phlebotomists play in the healthcare system and regularly writes content focused on blood collection best practices, troubleshooting common issues, and understanding the latest trends in phlebotomy equipment. Jessica aims to share practical insights and tips to help phlebotomists enhance their skills and improve patient care.

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