Understanding the Correct Order of Draw for Blood Collection in the United States

Summary

  • Understanding the correct order of draw for blood collection is essential in a medical laboratory setting in the United States.
  • Following the proper order of draw helps prevent Cross-Contamination and ensures accurate Test Results.
  • The order of draw typically involves collecting blood samples in tubes in a specific sequence to maintain the integrity of the specimens.

Introduction

When it comes to drawing blood for testing in a medical laboratory setting, following the correct order of draw is crucial. The order of draw refers to the sequence in which blood samples are collected into different tubes to prevent Cross-Contamination and ensure accurate Test Results. In the United States, Healthcare Providers, clinical lab technicians, and phlebotomists must adhere to the established order of draw guidelines to maintain the integrity of blood specimens. Let's explore the correct order of draw in blood collection and its importance in healthcare settings.

Why is the Order of Draw Important?

The order of draw is important for several reasons:

  1. Preventing Cross-Contamination: By following a specific order of draw, the risk of contaminating the blood samples with additives from other tubes is minimized. This helps ensure that the Test Results are accurate and reliable.
  2. Preserving Specimen Integrity: Certain additives in blood collection tubes can interfere with the accuracy of specific tests. Following the correct order of draw helps maintain the integrity of the specimens and ensures that the Test Results are not compromised.
  3. Complying with industry standards: Healthcare Providers and laboratory professionals are required to follow established guidelines and best practices for blood collection to maintain the quality of patient care and adhere to regulatory standards.

The Correct Order of Draw for Blood Collection

While there may be variations in the specific order of draw depending on the laboratory and the testing requirements, the following sequence is a commonly accepted guideline for blood collection in the United States:

1. Blood Cultures

Blood Culture bottles should always be collected first to prevent contamination of the samples with skin flora.

2. Blue-Top Tubes (Sodium Citrate)

These tubes are typically used for coagulation studies. Collecting them second helps prevent Cross-Contamination with anticoagulants from other tubes.

3. Red-Top Tubes (No Additive)

These tubes are used for various tests, including serum chemistry and blood bank studies. Collecting them after blue-top tubes helps prevent contamination with anticoagulants.

4. Gold or Red/Grey Tiger Top Tubes (Serum Separator Tube)

These tubes contain a gel that separates the serum from the blood cells. They are often used for chemistry tests. Collecting them after red-top tubes helps ensure proper separation of serum.

5. Green-Top Tubes (Heparin)

These tubes are commonly used for chemistry tests that require plasma. Collecting them after serum separator tubes avoids contamination with clot activators.

6. Lavender-Top Tubes (EDTA)

These tubes contain EDTA, which is an Anticoagulant used for various hematology tests. Collecting them after green-top tubes helps prevent carryover of Heparin.

7. Gray-Top Tubes (Oxalate/Fluoride)

These tubes are used for glucose testing. Collecting them last reduces the risk of contamination with other additives.

Conclusion

Understanding the correct order of draw for blood collection is essential for Healthcare Providers and laboratory professionals in the United States. By following the established guidelines, healthcare facilities can ensure the accuracy and reliability of Test Results, prevent Cross-Contamination, and maintain the integrity of blood specimens. Adhering to the proper order of draw is a fundamental aspect of quality patient care and regulatory compliance in clinical lab settings.

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