Understanding the Different Additives in Green Top Tubes for Blood Collection

Summary

  • Green top tubes are commonly used in the United States for blood collection
  • They contain additives that help with different types of blood tests
  • The most common additives found in green top tubes include lithium Heparin, sodium Heparin, and EDTA

Introduction

In the United States, green top tubes are frequently used for blood collection in various healthcare settings including home healthcare, clinical labs, and hospitals. These tubes are designed to contain additives that help preserve the quality of the blood sample and facilitate accurate Test Results. In this article, we will explore the different additives commonly found in green top tubes and their purposes in the blood collection process.

Types of Additives in Green Top Tubes

Lithium Heparin

Lithium Heparin is a common additive found in green top tubes used for blood collection. Heparin is an Anticoagulant that prevents blood from clotting, allowing for the preservation of the blood sample for various tests. Lithium Heparin provides a consistent Anticoagulant effect and is suitable for a wide range of laboratory tests including chemistry and immunology assays.

Sodium Heparin

Another type of Heparin commonly found in green top tubes is sodium Heparin. Similar to lithium Heparin, sodium Heparin acts as an Anticoagulant to prevent blood clotting and ensure the quality of the blood sample. Sodium Heparin is preferred for certain tests that require a different type of Anticoagulant or when lithium interference needs to be avoided.

EDTA (Ethylenediaminetetraacetic Acid)

EDTA is a chelating agent that is often used as an Anticoagulant in green top tubes for blood collection. It works by binding to calcium ions in the blood, preventing clotting and preserving the sample for tests that require whole blood or plasma. EDTA is commonly used in hematology and immunohematology tests that require a complete blood count (CBC) or blood typing and crossmatching.

Other Additives

In addition to lithium Heparin, sodium Heparin, and EDTA, green top tubes may also contain other additives depending on the specific laboratory tests that will be conducted. These additives could include preservatives, stabilizers, or buffers that help maintain the integrity of the blood sample and ensure accurate Test Results.

Uses of Green Top Tubes

  1. Chemistry Tests: Green top tubes with lithium Heparin or sodium Heparin are commonly used for chemistry tests such as glucose, electrolytes, and liver function tests.
  2. Hematology Tests: Green top tubes with EDTA are preferred for hematology tests including CBC, white blood cell differential, and red blood cell morphology.
  3. Immunology Tests: Green top tubes with lithium Heparin are suitable for immunology tests such as hormone assays, tumor markers, and Allergy Testing.
  4. Coagulation Tests: Green top tubes with sodium Heparin are used for coagulation tests including Prothrombin Time (PT), Activated Partial Thromboplastin Time (aPTT), and fibrinogen levels.

Conclusion

Green top tubes are essential components in blood collection processes in the United States, used in various healthcare settings for a wide range of laboratory tests. The additives found in green top tubes play a crucial role in preserving the quality of blood samples and ensuring accurate Test Results. Understanding the different types of additives in green top tubes and their uses is important for healthcare professionals to effectively collect and handle blood samples for laboratory testing.

a-phlebotomist-demonstrates-how-to-collect-blood

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