Key Tests and Tube Colors for Successful Phlebotomy Procedures in the United States
Summary
- Understanding the recommended order of draw is crucial for successful phlebotomy procedures in the United States.
- Following the correct order can help prevent sample contamination and ensure accurate Test Results.
- Key tests and corresponding tube colors for each step in the order of draw must be closely followed.
Introduction
Phlebotomy is a critical component of healthcare, involving the collection of blood samples for diagnostic testing. To ensure accurate results, phlebotomists must follow a specific order of draw when collecting blood samples. This article will explore the recommended order of draw and corresponding tests for a phlebotomy procedure in the United States.
Order of Draw
The order of draw refers to the sequence in which blood samples are collected during a phlebotomy procedure. Following this order helps prevent sample contamination and ensures accurate Test Results. The recommended order of draw in the United States is as follows:
1. Blood Cultures
First and foremost, blood cultures should be collected to detect any potential infections. This step helps prevent contamination of other tubes with bacteria present in the skin.
2. Light Blue Top Tubes (Sodium Citrate)
The next step involves collecting blood samples in Light Blue Top tubes, which contain Sodium Citrate as an anticoagulant. These tubes are used for coagulation studies, including Prothrombin Time (PT) and Activated Partial Thromboplastin Time (aPTT).
3. Red Top Tubes (No Additive)
Red Top tubes without additives are used for collecting serum samples. These tubes are commonly used for various tests, including blood chemistries, lipid profiles, and blood type and crossmatch.
4. Gold or Tiger Top Tubes (Serum Separator Tubes)
Gold or tiger top tubes contain a gel separator that separates the serum from the blood after centrifugation. These tubes are used for tests such as comprehensive metabolic panels (CMP) and liver function tests.
5. Light Green Top Tubes (Heparin)
Light Green Top tubes contain Heparin as an Anticoagulant and are used for tests such as electrolyte panels and some coagulation studies.
6. Lavender Top Tubes (EDTA)
Lavender Top tubes contain EDTA (ethylenediaminetetraacetic acid) as an Anticoagulant and are used for hematology tests, such as complete blood count (CBC) and blood cell morphology.
7. Gray Top Tubes (Potassium Oxalate/Sodium Fluoride)
Gray top tubes contain Potassium Oxalate and Sodium Fluoride as additives to preserve glucose levels in the sample. These tubes are used for glucose testing, such as Fasting blood sugar levels.
8. Dark Blue Top Tubes (Sodium Heparin)
Dark Blue Top tubes contain sodium Heparin as an Anticoagulant and are used for Toxicology and Drug Level Monitoring tests.
Conclusion
Following the recommended order of draw is essential for conducting successful phlebotomy procedures in the United States. By adhering to this order, phlebotomists can minimize the risk of sample contamination and ensure accurate Test Results. Understanding the key tests and corresponding tube colors for each step in the order of draw is crucial for providing high-quality patient care.
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