Order of Draw for Phlebotomy Procedures: Understanding the Importance of Each Tube

Summary

• Understanding the order of draw for phlebotomy procedures is crucial for accurate Test Results
• Each tube of blood collected serves a specific purpose in the testing process
• Compliance with testing requirements ensures patient safety and quality care

Introduction

Phlebotomy procedures are an essential part of healthcare, especially in the context of home healthcare, clinical labs, and hospitals in the United States. Collecting blood samples for testing requires careful attention to detail, including following the correct order of draw for different types of tubes. In this blog post, we will explore the specific testing requirements for each tube of blood collected in the order of draw for phlebotomy procedures.

Order of Draw

The order of draw refers to the sequence in which different tubes are filled during a blood draw. This process is crucial for ensuring the accuracy of Test Results and preventing sample contamination. Each tube is designed for specific types of tests and additives, which is why following the correct order is vital.

Specific Testing Requirements for Each Tube

Below is a breakdown of the order of draw for phlebotomy procedures and the specific testing requirements for each tube:

1. Yellow Tube (SPS)

   This tube contains Sodium Polyanethol Sulfonate (SPS) and is used for blood cultures. It is important to draw this tube first to prevent contamination from other additives.

2. Light Blue Tube (Sodium Citrate)

   This tube contains Sodium Citrate and is used for coagulation studies, such as PT and PTT tests.

3. Red/Gray Tube (Serum Separator Tube)

   This tube contains a gel separator and is used for various chemistry tests, such as glucose, cholesterol, and electrolytes.

4. Red Tube (No Additive)

   This tube does not contain any additives and is used for serum testing, including drug levels, hormones, and antibodies.

5. Gold Tube (Serum Separator Gel with Clot Activator)

   This tube contains a gel separator and a Clot Activator, making it suitable for chemistry tests and some immunology tests.

6. Green Tube (Heparin)

   This tube contains Heparin and is used for plasma determinations, such as electrolytes, ammonia, and some drug levels.

7. Lavender Tube (EDTA)

   This tube contains EDTA and is used for hematology studies, such as CBC and Blood Smears.

8. Gray Tube (Oxalate/Fluoride)

   This tube contains Oxalate and Fluoride and is used for glucose testing, as the additives stabilize Blood Glucose levels.

Conclusion

Understanding the specific testing requirements for each tube of blood collected in the order of draw is essential for phlebotomy procedures in the United States. Following the correct sequence and using the appropriate tubes for different tests ensures accurate results and patient safety. Compliance with testing requirements is paramount in home healthcare, clinical labs, and hospitals to provide quality care and contribute to positive healthcare outcomes.

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