Common Mistakes in Phlebotomy Order of Draw: How to Avoid Blood Sample Contamination

Summary

• Incorrect order of draw can lead to blood sample contamination
• Using the correct order of draw ensures accurate Test Results
• Common mistakes can be easily avoided with proper training and attention to detail

Phlebotomy is a crucial aspect of healthcare, as it involves drawing blood samples for various Diagnostic Tests. One important factor in phlebotomy is the order of draw, which refers to the sequence in which different color-coded tubes should be filled with blood. Failure to follow the correct order of draw can result in sample contamination and inaccurate Test Results. In this article, we will discuss some of the common mistakes made in phlebotomy order of draw and how to avoid them.

Mistake #1: Not Following the Correct Order

One of the most common mistakes in phlebotomy is not following the correct order of draw. Each color-coded tube is designed to contain specific additives that prevent blood clotting and preserve the integrity of the sample. Failing to fill the tubes in the correct order can lead to contamination and inaccurate Test Results.

How to Avoid:

1. Always refer to the standard phlebotomy order of draw chart provided by your facility.
2. Double-check the color-coded tubes and their specific additives before starting the blood draw.
3. Follow a consistent and systematic approach to ensure the correct order of draw is maintained.

Mistake #2: Inadequate Mixing of Tubes

After collecting blood into the appropriate tubes, it is essential to mix the samples thoroughly to ensure proper distribution of additives. Inadequate mixing can lead to improper clotting times or incomplete preservation of the sample, resulting in inaccurate Test Results.

How to Avoid:

1. Gently invert each tube for the recommended number of times as per the manufacturer's instructions.
2. Avoid vigorous shaking or agitation, as this can cause hemolysis and compromise the sample quality.
3. Ensure proper mixing before sending the tubes to the laboratory for analysis.

Mistake #3: Using Expired or Damaged Tubes

Another common mistake in phlebotomy is using expired or damaged tubes for blood collection. Expired tubes may have compromised additives or vacuum seal, leading to unreliable Test Results. Similarly, using tubes with visible cracks or defects can result in sample contamination and Specimen Rejection.

How to Avoid:

1. Check the expiration date of each tube before use and discard any expired tubes.
2. Inspect tubes for any visible damage, such as cracks, leaks, or discoloration, and replace them as necessary.
3. Store tubes properly in a cool, dry place to maintain their integrity and ensure reliable Test Results.

Phlebotomy order of draw is a critical aspect of blood collection that directly impacts the quality and accuracy of diagnostic Test Results. By avoiding common mistakes such as not following the correct order, inadequate mixing of tubes, and using expired or damaged tubes, phlebotomists can ensure reliable and consistent sample collection. Proper training, attention to detail, and adherence to best practices are essential in preventing errors and maintaining the integrity of blood samples in phlebotomy.

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