Understanding the Order Of Draw Is Crucial For Accurate Blood Collection

Summary

• Understanding the order of draw is crucial for accurate blood collection.
• There are several different types of tubes used in the order of draw, each serving a specific purpose.
• It is important for healthcare professionals to be knowledgeable about the different tubes and their uses to ensure the integrity of blood samples.

Introduction

When it comes to blood collection, following the proper order of draw is essential for ensuring accurate results. Different types of tubes are used for collecting blood samples, each serving a specific purpose in the laboratory testing process. In this article, we will explore the various tubes used in the order of draw and their significance in the collection and analysis of blood samples.

Types of Tubes Used in the Order of Draw

1. Yellow Tube (Sterile Tube)

The first tube used in the order of draw is the yellow tube, also known as the sterile tube. This tube contains the Anticoagulant solution ACD (acid citrate dextrose) and is used for collecting blood cultures. Blood cultures are collected to detect the presence of bacteria or fungi in the bloodstream, which can indicate an infection.

2. Light Blue Tube

The light blue tube is the second tube used in the order of draw and contains the Anticoagulant solution Sodium Citrate. This tube is used for coagulation studies, including Prothrombin Time (PT) and Activated Partial Thromboplastin Time (aPTT) tests. These tests evaluate the blood's ability to clot and are used to diagnose and monitor bleeding disorders, Clotting Disorders, and to assess the effectiveness of Anticoagulant therapy.

3. Red Tube (Plain Tube)

The red tube, also known as the plain tube, does not contain any additives and is used for collecting serum samples. Serum is the liquid portion of blood that remains after the blood has clotted. These tubes are used for a wide range of tests, including chemistry panels, lipid profiles, and hormone tests.

4. Tiger/Gold Tube (Serum Separator Tube)

The tiger or gold tube, also known as the serum separator tube, contains a gel separator that separates the serum from the clot when centrifuged. These tubes are used for similar tests as the red tube, but the gel separator allows for easier serum separation, reducing the risk of hemolysis (rupture of red blood cells) and improving Sample Integrity.

5. Green Tube

The green tube contains the Anticoagulant solution Heparin and is used for collecting plasma samples. Heparin prevents blood from clotting by inhibiting thrombin, an enzyme necessary for clot formation. Plasma is the liquid portion of blood that remains after the blood has been centrifuged and separated from the cells. These tubes are used for tests such as glucose, electrolytes, and blood gases.

6. Lavender Tube

The lavender tube contains the Anticoagulant solution EDTA (ethylenediaminetetraacetic acid) and is used for collecting whole blood samples for hematological tests. EDTA prevents blood from clotting by binding to calcium, which is necessary for the clotting process. These tubes are used for complete blood counts (CBC), blood typing, and other hematological tests.

7. Gray Tube

The gray tube contains the Anticoagulant solution Sodium Fluoride and an anticoagulant/preservative solution, Potassium Oxalate. This tube is used for collecting blood samples for glucose testing. Sodium Fluoride inhibits glycolysis (the breakdown of glucose) in the blood sample, ensuring accurate glucose measurements. The Potassium Oxalate acts as an Anticoagulant by binding to calcium and preventing blood from clotting.

Conclusion

Understanding the different tubes used in the order of draw is crucial for healthcare professionals involved in blood collection and testing. By following the proper order of draw and using the appropriate tubes for each type of testing, they can ensure the accuracy and reliability of blood samples. Knowing the purpose and significance of each tube helps in maintaining Sample Integrity and obtaining precise Test Results, ultimately leading to better patient care and outcomes. Healthcare professionals should be well-versed in the different tubes used in the order of draw to facilitate efficient and effective blood collection and laboratory testing processes.

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